anti rat cd71 fitc  (Bio-Rad)

Journal: Animal Models and Experimental Medicine

Article Title: A rat model of multicompartmental traumatic injury and hemorrhagic shock induces bone marrow dysfunction and profound anemia

doi: 10.1002/ame2.12447

Figure Lengend Snippet: (A, B) Sample flow demonstrating impact of injury on CD117 + CD71 + hematopoietic stem cell mobilization. Peripheral red cell‐lysed blood cells labeled using APC rat anti‐mouse CD117 and FITC mouse anti‐rat CD71. (C) Circulating plasma hematopoietic stem cells. (D) plasma G‐CSF levels. LCHS, lung contusion and hemorrhagic shock; PT, polytrauma. Only statistically significant comparisons displayed (* p < 0.05).

Article Snippet: Briefly, 100 μL of whole blood was incubated with mouse anti‐rat CD71‐FITC (BD Biosciences, San Jose, CA, USA) along with rat anti‐mouse CD117‐APC (Invitrogen, Waltham, MA, USA) and run on a BD LSR II flow cytometer equipped with FACSDiva software (BD Biosciences) to quantitate CD117 + CD71 + cells.

Techniques: Labeling

Journal: Oncogene

Article Title: FANCA deficiency promotes leukaemic progression by allowing the emergence of cells carrying oncogenic driver mutations

doi: 10.1038/s41388-023-02800-9

Figure Lengend Snippet: A Schematic diagram representing erythroid differentiation with the specific phenotypic markers used. B Representative profiles of cells incubated with antibodies against Ter119 and CD71 and analysed by flow cytometry. C Histograms represent the mean % of each population for mice analysed (from 4 to 8 mice) according to Ter119 and CD71 markers and basophilic, polychromatic and acidophilic cells relative to 100% of bone marrow or spleen cells as described in A and B. Statistical analysis was carried out using the Mann‒Whitney test. Three types of comparisons were performed: FA −/− to TgSpi1FA −/− ; FA +/+ to TgSpi1FA +/+ and TgSpi1FA −/− to TgSpi1FA +/+ . For better visualization, only one * was written for all P values ( P < 0.05), even if more highly significant.

Article Snippet: Single-cell suspensions of freshly collected bone marrow cells were immunostained with APC-H7 (560185, BD Biosciences) or APCeFluor780 (eBioscience 47-1172-82) -conjugated rat anti-KIT, FITC-conjugated rat anti-CD71 (553266, BD Biosciences), APC-conjugated rat anti-Ter119 (557909, BD Biosciences) and PE-conjugated streptavidin (554061, BD Biosciences) and biotin-conjugated rat anti-CD123 (555070, BD Biosciences) antibodies for the detection of erythroid progenitor (CFU-E) cells, as previously described [ ]; FITC-conjugated rat anti-CD11b (553310, BD Biosciences), PerCP-Cy TM 5.5-conjugated rat anti-mouse CD19 (45-0193-82, eBioscience) and PE-conjugated rat anti-mouse CD4 (553049, BD Biosciences), and APC-conjugated rat anti-myeloid CD8 (553035, BD Biosciences) and PE-Cy TM 7- conjugated rat anti-myeloid B220 (25-0452-82, eBioscience) antibodies were used for the detection of freshly collected bone marrow cells.

Techniques: Incubation, Flow Cytometry